Hematology · LIVE

DLI process volume

Compute Shi 2020 predicted donor whole-blood volume (L) to process to reach a recipient CD3+ DLI target from recipient weight, CD3 cells/kg, donor collection-day ALC, and caller-assigned collection efficiency, using that paper's conservative 60% CD3 fraction of ALC.

LIVE $0.005 USDC GET /api/calc/dli_volume

Markdown: /docs/dli-volume.md · Canonical: https://magentlab.com/docs/dli-volume

Not a medical device. Not a diagnosis, dose, or listing recommendation.

What it computes

Compute Shi 2020 predicted donor whole-blood volume (L) to process to reach a recipient CD3+ DLI target from recipient weight, CD3 cells/kg, donor collection-day ALC, and caller-assigned collection efficiency, using that paper's conservative 60% CD3 fraction of ALC.

When to use

When an agent needs the published Shi 2020 DLI process-volume algorithm and must not invent a CD3 fraction, hardcode collection efficiency, write an apheresis order, or run the CD34 HPC algorithm from the same paper.

When not to use

• Not a medical device. magent does not run leukapheresis, write an apheresis order, choose a machine protocol, or set a collection goal.
• Uses only Shi 2020's conservative 60% CD3+ fraction of ALC. Does not accept a measured CD3 count. Not the paper's CD34 HPC or postselection algorithm.
• collection_efficiency is caller-assigned facility CE2 in (0, 1]. Shi 2020 used a conservative first-quartile 0.57; magent does not default to 0.57.
• Weight, CD3-dose, ALC, and CE clamps are fail-closed magent ranges (positive, clinically plausible). Shi 2020 does not print those input bands.
• num_infusions multiplies the per-kg target when the planned dose is the total across infusions. The paper's protocol collected one 2×10^8/kg DLI product.
• Not Rosenbaum CD34 yield, Kaplan plasma volume, or Nadler blood volume.

Formula

process_volume_l = (recipient_weight_kg * cd3_target_per_kg * num_infusions) / (0.6 * donor_alc_per_ul * collection_efficiency * 1e6)

Citation

Title

Validation of simple prediction algorithms to consistently achieve CD3+ and postselection CD34+ targets with leukapheresis

Authors

Shi PA, Sachais BS, Nandi V, Brochstein JA, Weinberg RS, Zhang J, Yoon EJ

Source

Transfusion. 2020;60(1):133-143

DOI

10.1111/trf.15576

Worked example

Also searched as

• DLI process volume
• donor lymphocyte infusion volume
• CD3 leukapheresis volume
• Shi DLI algorithm
• DLI blood volume to process
• tandem DLI collection volume
• CD3 target leukapheresis
• donor ALC DLI

Try

Opens the live endpoint. Unpaid browser requests show the paywall; agents should send Accept: application/json.

/api/calc/dli_volume?recipient_weight_kg=70&cd3_target_per_kg=10000000&donor_alc_per_ul=1800&collection_efficiency=0.5

Full URL: https://api.magentlab.com/api/calc/dli_volume?recipient_weight_kg=70&cd3_target_per_kg=10000000&donor_alc_per_ul=1800&collection_efficiency=0.5

Call

Expect HTTP 402 until you retry with PAYMENT-SIGNATURE. Incomplete query params return HTTP 400 before any quote or charge.

curl -i -H "Accept: application/json" "https://api.magentlab.com/api/calc/dli_volume?recipient_weight_kg=70&cd3_target_per_kg=10000000&donor_alc_per_ul=1800&collection_efficiency=0.5"

Parameters

Bulk (POST)

POST the same path with a JSON items array. The first item is the GET unit; each additional item is $0.002. Invalid items return HTTP 400 before settlement.

POST /api/calc/dli_volume Max 25 unit_amount + extra_item_amount * (n - 1) extra $0.002 PREVIEW

• 1 to 25 items. Each item uses the same keys as the GET query parameters.
• POST of 1 item costs the same as GET. Lists longer than 25: chunk into multiple POSTs. Extra items still execute; do not pad dummy rows.
• The first invalid item fails the whole request (index in the 400 JSON). You are not charged.
• HTTP 200 is {count, path, items}. Each items[i] is the GET 200 body for that row.
• Catalog misses (empty matches, found=false) stay paid 200s inside that slot.
• x402 resource URL is this path plus n and body_sha256. Browsers that prefer text/html get the same paywall as GET; Pay retries POST with the same items.

Example request (fixture)

{
  "items": [
    {
      "cd3_target_per_kg": "10000000",
      "collection_efficiency": "0.5",
      "donor_alc_per_ul": "1800",
      "recipient_weight_kg": "70"
    },
    {
      "cd3_target_per_kg": "10000000",
      "collection_efficiency": "0.5",
      "donor_alc_per_ul": "1800",
      "recipient_weight_kg": "70"
    }
  ]
}

Open bulk paywall →

Opens a two-item fixture on the API host. Unpaid browsers get the same MetaMask paywall as GET; Pay retries POST with those items.

Example 200 (fixture)

{
  "count": 2,
  "path": "/api/calc/dli_volume",
  "items": [
    {
      "disclaimer": "Not a medical device. Deterministic published-formula or published-code output for autonomous agents. A licensed clinician remains responsible for patient care and billing submissions.",
      "citation": {
        "authors": "Shi PA, Sachais BS, Nandi V, Brochstein JA, Weinberg RS, Zhang J, Yoon EJ",
        "doi": "10.1111/trf.15576",
        "id": "shi-2020-dli",
        "pmid": "31756000",
        "source": "Transfusion. 2020;60(1):133-143",
        "title": "Validation of simple prediction algorithms to consistently achieve CD3+ and postselection CD34+ targets with leukapheresis"
      },
      "formula": "shi_2020",
      "formula_expression": "process_volume_l = (recipient_weight_kg * cd3_target_per_kg * num_infusions) / (0.6 * donor_alc_per_ul * collection_efficiency * 1e6)",
      "cd3_fraction": 0.6,
      "cd3_target_per_kg": 1.0e7,
      "collection_efficiency": 0.5,
      "donor_alc_per_ul": 1.8e3,
      "estimated_cd3_per_ul": 1080.0,
      "num_infusions": 1,
      "process_volume_l": 1.3,
      "recipient_weight_kg": 70.0,
      "total_cd3_required": 7.0e8
    },
    {
      "disclaimer": "Not a medical device. Deterministic published-formula or published-code output for autonomous agents. A licensed clinician remains responsible for patient care and billing submissions.",
      "citation": {
        "authors": "Shi PA, Sachais BS, Nandi V, Brochstein JA, Weinberg RS, Zhang J, Yoon EJ",
        "doi": "10.1111/trf.15576",
        "id": "shi-2020-dli",
        "pmid": "31756000",
        "source": "Transfusion. 2020;60(1):133-143",
        "title": "Validation of simple prediction algorithms to consistently achieve CD3+ and postselection CD34+ targets with leukapheresis"
      },
      "formula": "shi_2020",
      "formula_expression": "process_volume_l = (recipient_weight_kg * cd3_target_per_kg * num_infusions) / (0.6 * donor_alc_per_ul * collection_efficiency * 1e6)",
      "cd3_fraction": 0.6,
      "cd3_target_per_kg": 1.0e7,
      "collection_efficiency": 0.5,
      "donor_alc_per_ul": 1.8e3,
      "estimated_cd3_per_ul": 1080.0,
      "num_infusions": 1,
      "process_volume_l": 1.3,
      "recipient_weight_kg": 70.0,
      "total_cd3_required": 7.0e8
    }
  ]
}

Response

Example JSON

Machine form of the same example. Frozen fixture. Not a live lookup.

{
  "disclaimer": "Not a medical device. Deterministic published-formula or published-code output for autonomous agents. A licensed clinician remains responsible for patient care and billing submissions.",
  "citation": {
    "authors": "Shi PA, Sachais BS, Nandi V, Brochstein JA, Weinberg RS, Zhang J, Yoon EJ",
    "doi": "10.1111/trf.15576",
    "id": "shi-2020-dli",
    "pmid": "31756000",
    "source": "Transfusion. 2020;60(1):133-143",
    "title": "Validation of simple prediction algorithms to consistently achieve CD3+ and postselection CD34+ targets with leukapheresis"
  },
  "formula": "shi_2020",
  "formula_expression": "process_volume_l = (recipient_weight_kg * cd3_target_per_kg * num_infusions) / (0.6 * donor_alc_per_ul * collection_efficiency * 1e6)",
  "cd3_fraction": 0.6,
  "cd3_target_per_kg": 1.0e7,
  "collection_efficiency": 0.5,
  "donor_alc_per_ul": 1.8e3,
  "estimated_cd3_per_ul": 1080.0,
  "num_infusions": 1,
  "process_volume_l": 1.3,
  "recipient_weight_kg": 70.0,
  "total_cd3_required": 7.0e8
}

Client errors (HTTP 400)

Invalid params return 400 without charge. Shared HTTP 402 and 503 meanings: Payments.

Related tools

• predicted CD34+ leukapheresis yield — Compute Rosenbaum 2012 predicted daily CD34+ yield (×10^6 per kg) from pre-apheresis peripheral blood CD34+ cells/µL, processed blood volume, and recipient or patient weight, using that paper's adjusted collection efficiency of 30%.
• Kaplan plasma volume — Compute Kaplan 1990 estimated plasma volume for therapeutic plasma exchange from weight and hematocrit as a percent, returning one plasma volume in liters and milliliters.
• Nadler blood volume — Compute Nadler 1962 predicted blood volume in liters and milliliters from height, weight, and sex.

Payment

Unpaid requests return HTTP 402 even if query parameters or POST items are missing or invalid, so CDP and agents can index the path. After a PAYMENT-SIGNATURE, invalid params return HTTP 400 JSON before settlement. magent does not charge. Fix the params, then request a new 402 quote for that complete URL. Catalog misses (empty matches, found=false) are valid paid 200s. Unpaid valid requests return HTTP 402 with a base64 PAYMENT-REQUIRED header. HTTP 503 is not a new quote: retry the same PAYMENT-SIGNATURE. Full handshake and shared payment errors: Payments. Stdio MCP: /docs/mcp.

Guarantees

• HTTP 402 is unpaid, including a missing query. After PAYMENT-SIGNATURE, invalid parameters return HTTP 400 before settlement. You are not charged. HTTP 402 never includes the tool JSON.
• exact: settlement finishes before the tool (paymentFlow upfront). batch-settlement: verify/deposit before the tool; chargedCumulativeAmount commits after HTTP 2xx. Uncertain or failed settlement fails closed (HTTP 503). Retry the same PAYMENT-SIGNATURE.
• HTTP 402 is unpaid only. Do not sign a second authorization because of a 503.
• Execution is timeout-bounded and concurrency-capped.
• Calculator inputs are stored encrypted for allowlisted operators (Magent Console). They are not in public logs, SSH lookups, catalog, or rollups. Request logs store path without query.